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WITNESS FFH

Introducing WITNESS FFH

TRIPLE THREAT

DETECT THREE CRITICAL DISEASES WITH ONE SIMPLE, CONVENIENT, AND COST-EFFECTIVE TEST

WITNESS® FFH is a triple test kit that detects antigens to feline leukemia (FeLV) and feline heartworm and antibodies to feline immunodeficiency virus (FIV).

STUDIES DEMONSTRATE RELIABLE PERFORMANCE

  • FeLV: 97.7% sensitivity* and 100% specificity1†
  • FIV: 95.2% sensitivity and 98.2% specificity2
  • Heartworm: 96.3% sensitivity and 100% specificity3

GET MORE VALUE IN A TRIPLE TEST

WITNESS FFH is the affordable and easy-to-use alternative to other dual FeLV-FIV brands on the market with the added benefit of offering heartworm testing.

WITNESS SIMPLICITY

WITNESS FFH uses only a single buffer, so there is no confusion with which buffer to use. And you can test for three—not just two—important feline diseases.

CONFIDENTLY TAKE ON THE TRIPLE THREAT OF FELV, FIV, AND HEARTWORM.

TEST CATS FOR HEARTWORM?4,5

Yes. Heartworm infection in cats is very different than in dogs, but it still has the potential to be just as deadly. Signs of heartworm infection in cats can be confused with signs of many other diseases, including feline asthma. Affected cats may vomit, cough, or have difficulty breathing.

In cats, the disease is caused by an inflammatory reaction to the presence of an adult heartworm or, more often, the immature adult worms. Unfortunately, the lungs of cats are profoundly reactive to the arrival or death of the immature or adult worms, such that significant morbidity and even death can occur.

WITNESS FFH VERSUS IDEXX SNAP® FELINE TRIPLE TEST*

  WITNESS FFH   IDEXX SNAP FELINE TRIPLE TEST
STORAGE No refrigeration required. vs. Refrigeration required.
PREP None required. vs. Allow time for components to warm to room temperature before use. Mixing of sample required before it can be added to the test.
TESTING Add buffer and get results in approximately 10 minutes. vs. Requires time-sensitive manipulation (snapping) before waiting for results.
RESULTS Simple-to-interpret results: one line for negative and two lines for positive. vs. Results are shown in a series of dots that may be more difficult to interpret.

DATA FROM STUDIES COMPARING PERFORMANCES OF WITNESS® FFH VS. IDEXX SNAP® FELINE TRIPLE TEST6,7

DATA FROM STUDIES COMPARING PERFORMANCES OF WITNESS® VS. IDEXX SNAP® FELINE TRIPLE

*Sensitivity represents the ability to correctly identify positive samples.
†Specificity represents the ability to correctly identify negative samples.
*Based on manufacturer supplied direction insert.

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  • Rapid Results
    Get results in your clinic in approximately 10 minutes for confident, timely treatment decisions.

    Simple Test Procedure
    Easy 2-step test procedure with fast results—just add sample and buffer.

    No Refrigeration
    WITNESS FFH can be stored at room temperature for 12 months from the manufacturing date.

    Backed by Zoetis Service and Support

    • Same level of customer support you’re accustomed to receiving.
    • Conveniently packaged in kits of 10 tests.
    PDF DOWNLOAD:
    WITNESS FFH Product Sheet
  • Sample Information
    The test can be performed with plasma, serum, or anticoagulated whole blood.

    • Always collect samples with a sterile needle and syringe.
    • Hemolysis does not significantly interfere with the test, but strongly hemolyzed samples may partly obscure a weak positive band.

    Step 1 2 3Instructions for Use

    1. SAMPLE APPLICATION
      • Tear open a pouch and place the test on a flat horizontal surface.
      • Holding the provided pipette vertically, add one drop of sample to each test well.
    2. BUFFER DISPENSING
      • Check that the sample has fully absorbed into the membrane.
      • Remove the cap from the buffer bottle, hold it vertically, and add two drops of buffer to each test well.
      • Leave the test device flat during migration of the sample/reagent complex through the reading windows.
    3. READING TEST
      • After 10 minutes, observe the presence or absence of pink/red bands in the reading windows (T) and (C).

    Note:

    • It is possible to read the test before 10 minutes if two pink/red bands are clearly visible in the results windows (T) and (C).
    • The presence of only one band in control window (C) prior to the end of the development time (10 minutes) does not mean that the test is complete, as a test band may appear more slowly than the control band.

    Important: Allow samples and buffer drops to fall onto the membrane in each sample window. Do not touch pipette tip, buffer bottle tip or the sample or buffer drops directly to the membrane.

    WITNESS FFH "How to" video

    PDF DOWNLOAD:
    WITNESS FFH Directional Insert
  • WITNESS FFH is a simple test based on Rapid Immuno Migration (RIM) technology. Feline Heartworm antigen is detected using anti-heartworm antibodies. FeLV antigen is detected using antibodies directed against the circulating p27 capsid protein. FIV antibody is detected based on the presence of antibodies against the transmembrane region of the virus, using a synthetic peptide from the gp40 protein.

    In all three cases, sensitized particles bound to the targeted analyte present in the sample (whole blood, serum, or plasma) migrate along a membrane.

    The complex is then captured on a sensitized reaction zone where its accumulation causes the formation of a clearly visible pink/red band.

    A control band located at the end of the reading window (C) ensures that the test was performed correctly.

    A test result should always be interpreted in the context of all available clinical information and history for the cat being tested.

Precautions

  • Do not use this kit or any of its components after expiration date.
  • Kit should be stored at 35˚ to 77˚F (2˚ to 25˚C). Kit should not be frozen.
  • Use the test within 10 minutes of opening the foil pouch.
  • Avoid touching or damaging the membrane in the sample wells or the results window.
  • The WITNESS device should be placed on a flat, horizontal surface while performing the test.
  • Use a separate pipette for each sample.
  • Hold pipette and buffer bottle vertically when dispensing sample and buffer, respectively.
  • Handle all samples as biohazardous material.
  • Saline buffer is preserved with sodium azide.
  • For veterinary use only.

References:

  1. Data on file, Study Report No. D886R-US-17-038, Zoetis Inc.
  2. Data on file, Study Report No. D886R-US-16-033, Zoetis Inc.
  3. Data on file, Study Report No. D886R-US-16-032, Zoetis Inc.
  4. Browne LE, Carter TD, Levy JK, et al. Pulmonary arterial disease in cats seropositive for Dirofilaria immitis but lacking adult heartworms in the heart and lungs. Am J Vet Res. 2005; 66:1544-1549.
  5. Lin CH, Lo PY, Tsai HJ, et al. Dirofilaria immitis exposure status in client-owned cats with or without lower airway/lung-associated signs: case-control study in a canine heartworm-endemic area. J Feline Med Surg. 2017;19(2):153-157.
  6. Data on file, Study Report No. D880W-US-17-042, Zoetis Inc.
  7. Data on file, Study Report No. D886Z-US-17-043, Zoetis Inc.

©2017 Zoetis Services LLC. All rights reserved. The product information provided in this site is intended only for residents of the United States. The products discussed herein may not have marketing authorization or may have different product labeling in different countries. The animal health information contained herein is provided for educational purposes only and is not intended to replace discussions with an animal healthcare professional. All decisions regarding the care of a veterinary patient must be made with an animal healthcare professional, considering the unique characteristics of the patient. WIT-00269

WITNESS FFH - "How To" video