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ProFLOK® HEV ELISA Hemorrhagic Enteritis Virus (HEV) Antibody Test Kit

Reliable detection of HEV antibodies in turkey flocks

An ELISA test kit for the detection of HEV antibodies in turkey serum

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  • Good Specificity and Sensitivity to HEV Antibodies 1

    • No False Positive Reactions
      • When tested with a monospecific turkey serum panel consisting of National Veterinary Services Laboratories (NVSL) Panel #2 and other turkey sera from the Ohio State University.
      • Each sample was tested with 6 replicates per plate and tested in 5 ProFLOK® HEV ELISA plates.

    1Data on file, Efficacy Study Report, July 27, 1992, Zoetis Inc.

  • Description: USDA-licensed, enzyme-linked immunosorbent assay (ELISA) screening test kit for the detection of Hemorrhagic Enteritis Virus (HEV) antibodies in turkey serum samples.

    Antibodies detected to: Hemorrhagic Enteritis Virus (HEV)

    Use Situations: Presumptive sero-diagnosis of HEV infection in unvaccinated turkey flocks:

    Collect 30 or more serum samples from clinically affected (acute phase) flocks and assay with the ProFlok HEV ELISA kit. Re-bleed clinically affected flocks 14 to 21 days after first serum collection (convalescent phase).

    Compare the acute and convalescent serum sample results. An increase of three ELISA titer groups or greater between the acute and convalescent phases, is suggestive of HEV infection.

    NOTE: Additional information such as flock history, clinical signs, necropsy findings, histopathologic evaluation, etc. are needed for a confirmed diagnosis of HEV infection.

    HEV vaccination evaluation:

    Collect and assay 30 serum samples per each turkey flock receiving HEV vaccine as follows:

    • 1 to 3 days of age and 10 to 14 days of age (measures maternal HEV antibody levels)

    • The day of vaccination (pre-vaccination)

    • 3 to 5 weeks post-vaccination

    • 7 to 9 weeks post-vaccination

    Sampling:  

    Serum samples are used for testing.

    Performance1:

    Good Specificity

    Good Sensitivity

    Excellent Reproducibility

    Test Time: Results available in approximately 2 hours.

    Packaging:

    Up to 900 tests (10 plates of 96 wells)

    Store test kits at 2ºC – 7ºC (36ºF-45°F) (do not freeze)

    Long shelf life of 24 months for easy inventory management

    1Data on file, Efficacy Study Report, July 27, 1992, Zoetis Inc.

ProFLOK ELISA