Bovine Mycobacterium avium subspecies paratuberculosis Antibody Test Kit
SERELISA® ParaTB Ab Mono Indirect is a rapid and specific presumptive screening, well-based ELISA for the detection of antibody to Mycobacterium avium subspecies paratuberculosis specific antibodies (Johne’s disease).
Zoetis SERELISA ParaTB Ab Mono Indirect offers laboratories:
Excellent specificity and sensitivity1
Fast results – in approximately 75 minutes
An easy-to-use testing method
1 Data on file, Product Improvement Validation Report June 20, 2013, Zoetis Inc.
SERELISA® ParaTB Ab Mono Indirect Bovine Mycobacterium avium subspecies paratuberculosis Antibody Test Kit
The principle of the test is as follows: Serum obtained from bovine herds exposed to ParaTB antigens can contain specific ParaTB antibodies. Serum, diluted in Sample Diluent (containing Mycobacterium phlei extracts), is added to antigen coated wells. Specific antibody in the serum forms an antibody-antigen complex with the antigen bound to the well. After washing, a monoclonal antibovine IgG HRP conjugate is added to each well. The antibody-antigen complex remaining from the previous step binds with the conjugate. After a brief incubation period and a second wash, any unbound conjugate is removed. Substrate, which contains a chromagen (ABTS), is added to each well. Chromagen color change (from clear to green-blue) occurs in the presence of the HRP enzyme. After the substrate has incubated, Stop Solution is added to each well to terminate the reaction and the plate is read using an ELISA plate reader at 405-410 nm. The relative intensity of color developed in 15 minutes (compared to controls) is directly proportional to the level of antibody in the serum.