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PROOF THAT EACH GENERATION GETS BETTER

THE 3RD GENERATION TEST THAT DELIVERS ACCURATE RESULTS ACROSS MULTIPLE POPULATIONS IN AN EASY-TO-USE FORMAT

Accurate – Provides results comparable to IDEXX SNAP® 4DX1

Using DiroCHEK® as a reference method

DiroCHEK® is an Enzyme-Linked Immunosorbent Assay (ELISA) for the detection of antigen to adult Dirofilaria immitis antigen in canine anf feline plasma or serum.

*Sensitivity represents the ability to correctly identify positive samples.
Specificity represents the ability to correctly identify negative samples.

Simple - Faster and easier to use

Affordable

An affordable alternative to other point-of-care heartworm diagnostic tests

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  • No Refrigeration
    WITNESS® Heartworm has a 12-month shelf life at room temperature (versus only 90 days for a leading competitor’s test).

    No Snapping
    RIM™ (Rapid Immuno Migration) technology allows for a one-step process, as opposed to the ELISA method. So unlike with other in-house tests, you don’t lose time waiting to snap. Plus, you only need one drop of sample (anticoagulated whole blood, serum, or plasma).

    Rapid Results
    With accurate results in approximately minutes you can give your clients the immediate test results they want and get your patients on a heartworm preventive sooner.

    Easy to Interpret
    You get simple-to-understand results, with no complex interpretation needed:

    • One line on the right = negative
    • Two lines = positive

    Backed by Zoetis Service and Support

    • Same level of customer support you’re accustomed to receiving
    • Free confirmatory tests when appropriate www.zoetisus.com/vmips

    WITNESS® Heartworm Detail Aid

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    Watch the test procedure demonstration hereOpens in a new window

  • Sample Information

    All Drops Are Not Created Equal Detail AidOpens in a new window

    A sample size of 0.05 mL should be used. An appropriate sample can be any one of the following:

    • Anticoagulated whole blood (blood that has been collected in an EDTA [purple top] or heparin [green top] tube)
    • Serum
    • Plasma

    Instructions for Use

    1. Open the foil pouch and place the test kit on a flat horizontal surface for the duration of the test.
    2. Using the supplied pipette,* transfer onto the sample well (window 1), and allow for the sample to be completely absorbed.
    3. Add 2 drops of buffer to the sample well (window 1).
    4. Wait 10 minutes for sample and buffer to migrate across the test kit into windows 2 and 3.
    5. Read test results. When the control line appears in window 3, the test was performed correctly.

    *It is important to use the pipette provided to ensure enough sample is dropped into the well for accurate results. 

    • WITNESS® Heartworm detects the presence of adult Dirofilaria immitis antigens in dog or cat blood. The WITNESS® format is referred to as Rapid Immuno Migration (RIM). This technology is also sometimes referred to as lateral flow or rapid immunochromatography. WITNESS® tests perform well without refrigeration using an antigen-specific antibody labeled with colloidal gold.
    • After the sample and buffer are added to the sample pad (window 1), any antigens in the sample migrate across a nitrocellulose membrane along with the gold-labeled antibody (conjugate) and form an antigen/antibody gold complex. A second antibody specific to the antigen binds the antigen/antibody gold complex at the test line.
      • In a positive sample, this generates a pink to purple colored reaction line in window 2. Excess colloidal gold migrates across to the control line, where an antibody directed to the gold-labeled antibody binds to it. This causes formation of a colored control line (window 3).
    • A test result should always be interpreted in the context of all available clinical information and history for the dog or cat. Cats testing negative for heartworm antigen may benefit from other diagnostic tests, including heartworm antibody assays.

    WITNESS® Heartworm DI     WITNESS® Heartworm Antigen Test Kit Detailer

    WITNESS® Heartworm Technical Bulletin     WITNESS® Heartworm New Sell Sheet

    WITNESS® Heartworm Test Procedure
    WITNESS Heartworm Procedure

WITNESS® Heartworm Testimonial

WITNESS® Heartworm DEMO
EASY TO USE - SIMPLY SMART

WITNESS® Heartworm vs. IDEXX SNAP

WITNESS® Heartworm
A LOOK INSIDE THE TEST

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