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WITNESS Parvo

WITNESS® PARVO PROVIDES IMMEDIATE RESULTS SO YOUR CLIENTS CAN REST EASY

A CONVENIENT, EASY-TO-USE, IN-CLINIC TEST THAT DETECTS ALL CANINE PARVOVIRUS STRAINS SHED IN FECES.

WITNESS Parvo is a simple test, based on rapid immunomigration (RIM) technology, which detects the presence of canine parvovirus antigen in dog feces.

ACCURATE. AFFORDABLE. SIMPLE.

Experience the demonstrated accuracy1,2 and affordability of WITNESS Parvo test kits along with Zoetis service and support.

  • Get accurate results in your clinic in approximately 10 minutes for confident, timely treatment decisions
  • Easy test procedure with fast results
  • No time lost waiting to snap
  • Room temperature storage—ready when you are
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  • NO REFRIGERATION
    WITNESS Parvo has a 12-month shelf life at room temperature.

    NO SNAPPING
    Unlike other point-of-care tests, WITNESS Parvo doesn’t need to be snapped. No snapping, so no splashing.

    RAPID RESULTS
    With accurate results in approximately 10 minutes, you can give your clients immediate confirmation and allow for prompt quarantine and treatment of your patients.

    EASY TO INTERPRET
    You get simple-to-understand results, with no complex interpretation needed:

    • One line on the right = negative
    • Two lines = positive

    BACKED BY ZOETIS SERVICE AND SUPPORT

    PDF DOWNLOAD:
    Link to WITNESS PARVO PRODUCT INFO
  • Important: Allow sample drops to fall onto the membrane at window (1). Do not touch the device tip or the sample drops directly to the membrane.

    1. SAMPLE APPLICATION
      • Remove the top portion containing the cotton swab from a sample extraction device.
      • Dispense extraction buffer into the base of the sample extraction device until the buffer reaches the graduation mark.
      • Taking the top portion of the sample extraction device, coat the cotton swab with a thin layer of feces from a stool sample or a rectal swab.
      • Insert the swab into the dispensed extraction buffer in the base of the sample extraction device. Securely fit the top portion onto the base and tap to mix for 5 seconds to ensure good sample extraction.
    2. BUFFER DISPENSING
      • Tear open a pouch provided and place the test device on a flat, horizontal surface.
      • Snap off the top of the sample extraction device (at the blue line) containing the extracted fecal sample.
      • Invert and hold the sample extraction device in a vertical (upright) position and squeeze the base gently to dispense five drops of sample to the test well.
      • Leave the test device flat during migration of sample/reagent complex through the reading windows.
    3. READING TEST

      After 10 minutes, observe the presence or absence of pink/red bands in the reading windows (2) and (3).

    Note:

    • It is possible to read the test before 10 minutes if two pink/red bands are clearly visible in the results windows (2) and (3).
    • Wait a full 10 minutes before declaring a result as negative.
    • The presence of only one band in reading window (3), prior to the end of the development time (10 minutes), does not mean that the test is complete, as a test band may appear more slowly than the control band.

    RESULTS:

    Validation:
    Test is validated if a pink/purple band is present in window (3).

    Interpretation:

    • Negative for canine parvovirus (CPV) antigen: No band in window (2), with one pink/purple band in window (3).
    • Positive for CPV antigen: One pink/purple band in window (2), with one pink/purple band in window (3).
    • Samples with low levels of CPV antigen may produce a lighter colored yet distinct pink/purple band.

    Invalid test:
    No pink/purple band in window (3).

    NOTE:
    A test result should always be interpreted in the context of all available clinical information and history for the dog. If clinical signs warrant, it is recommended to retest using a fresh sample.

    PDF DOWNLOAD:
    Link to WITNESS PARVO Directional Insert
  •   WITNESS® PARVO   IDEXX SNAP® PARVO TEST*
    STORAGE No refrigeration required. vs. Refrigeration required.
    PREP Mixing of buffer and sample required. vs. Allow time for components to warm to room temperature before use. Mixing of sample and buffer required.
    TESTING Add sample and get results in approximately 10 minutes. vs. Requires time-sensitive manipulation (snapping) before waiting 8 minutes for results.
    RESULTS Simple-to-interpret results: one line for negative and two lines for positive. vs. Results are shown in a series of dots that may be more difficult to interpret.

    *Based on manufacturer directional insert.

  • Canine Parvovirus (CPV) is a member of the feline parvovirus subgroup.

    It is closely related to feline panleukopenia virus and mink enteritis virus, and is considered endemic to nearly all populations of domesticated and wild dogs.

    CPV causes two forms of disease: myocarditis and enteritis. Due to maternal antibody protection, the myocardial form is rare. The enteric form, however, is prevalent and can be fatal to puppies and geriatric dogs. CPV enteritis causes severe, often bloody diarrhea, vomiting, leukopenia and dehydration.

    Transmission is fecal-oral and most infections occur from exposure to contaminated feces. CPV is highly contagious and stable under a variety of environmental conditions. Rapid diagnosis of CPV allows for quarantine and prompt treatment of infected dogs. Diagnosis may be difficult in milder cases. WITNESS PARVO detects parvovirus strains 2a, 2b, and 2c shed in the feces.1,2 Positive results with WITNESS PARVO indicate the presence of canine parvovirus.

    Sensitivity 86.3% (95% CI: 76.6%-92.4%)2
    Specificity 96.1% (95% CI: 89.2%-98.7%)2
    N = 150 samples
    Reference test—hemagglutination (HA)

  • What is RIM™ Technology?

    Rapid Immuno Migration Technology, known as RIM™ Technology, allows for a one-step lateral flow process eliminating the need to snap.

    WITNESS test kits utilize the stability of colloidal gold conjugate to deliver a longer shelf life at room temperature and provide immediate and accurate results.

Precautions

  • Do not use this kit or any of its components after the expiration date.
  • Kit should be stored at +2°C-25°C (+35°F-77°F). Kit should not be frozen.
  • Use the test immediately after the pouch is first opened (within 10 minutes).
  • Avoid touching or damaging the membrane in the sample wells or the results windows.
  • The WITNESS device should be placed on a flat, horizontal surface while performing the test.
  • Use a separate extraction device for each sample.
  • Hold extraction device and buffer bottle vertically when dispensing sample and buffer, respectively.
  • Handle all samples as biohazardous material.
  • Extraction buffer is preserved with sodium azide.
  • For veterinary use only.

References:

  1. Lacheretz A, Laperrousaz C, Kdjo A, et al. Diagnosis of canine parvovirus by rapid immunomigration on a membrane. Vet Rec. 2003;152(2):48-50.
  2. Decaro N, Desario C, Billi M, et al. Evaluation of an in-clinic assay for the diagnosis of canine parvovirus. Vet J. 2013;198:504-507.

©2017 Zoetis Services LLC. All rights reserved. The product information provided in this site is intended only for residents of the United States. The products discussed herein may not have marketing authorization or may have different product labeling in different countries. The animal health information contained herein is provided for educational purposes only and is not intended to replace discussions with an animal healthcare professional. All decisions regarding the care of a veterinary patient must be made with an animal healthcare professional, considering the unique characteristics of the patient. WIT-00315