AGID-EIA is a high-performance agar gel immunodiffusion (AGID) test for the detection of antibodies to Equine Infectious Anemia (EIA) virus. Migration of antigen and antibody through agar gel results in formation of clearly defined precipitin lines to provide easy-to-interpret test results.
RELIABLY CLEAR ANTIGEN-ANTIBODY PRECIPITIN LINES TO DIFFERENTIATE BETWEEN POSITIVE AND NEGATIVE SAMPLES
AGID-EIA – THE EASY-TO-READ EIA TEST KIT AGID-EIA uses a highly purified recombinant protein from the EIA virus. A distinct line will form to identify antibodies in serum of EIA-infected horses. No precipitin lines will form if the serum is negative for antibodies to the EIA virus.
For two National Veterinary Services Laboratory (NVSL) proficiency panels of samples (n = 39) and of convenience, contemporaneous samples (n = 60) comparing the Zoetis AGID-EIA, the Veterinary Medical Research & Development (VMRD) and the IDEXX AGID EIA tests:
The Zoetis AGID-EIA and VMRD tests demonstrated results in agreement with NSVL-reported results.
The Zoetis AGID-EIA and VMRD tests produced identical results, correctly identifying all negative samples.
The majority of IDEXX tests could not be interpreted due to faint precipitin lines.
Results remain unchanged at both 24 and 48 hours for all tests.
Pare J, Simard C. Comparison of commercial enzyme-linked immunosorbent assays and agar gel immunodiffusion tests for the serodiagnosis of equine infectious anemia. Can J Vet Res. 2004;68:254-258.
Data on file. Study Report No. D850R-US-18-003, Zoetis Inc.